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sfGFP Antibody#48545

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Product Detail

Product NamesfGFP Antibody

ClonalityPolyclonal

PurificationPeptide affinity purified.

ApplicationsWB

Immunogen DescSynthetic peptide RDHMVLHEYVNAAGITC linked to KLH.

ConjugateUnconjugated

Concentration1mg/ml

Formulation1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Application Details

WB: 1:1,000

 

Western blot analysis of GFP11-tag on fusion protein using anti-GFP11-tag antibody at 1/1,000 dilution.

Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.

If you have published an article using product 48545, please notify us so that we can cite your literature.

et al,Enhancing mitochondrial proteolysis alleviates alpha-synuclein-mediated cellular toxicity. In NPJ Parkinsons Dis on 2024 Jun 21 by Xi Zhang, Linhao Ruan,et al..PMID:38906862 , (2024),
PMID: 38906862

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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