Product Detail
Product NameRas Rabbit mAb
Clone No.SA39-05
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, IP, FC
Species ReactivityHu, Ms, Rt, zebrafish
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesC-BAS/HAS antibody
c-H-ras antibody
C-HA-RAS1 antibody
CTLO antibody
GTPase HRas antibody
GTPase KRas antibody
GTPase NRas antibody
H-Ras-1 antibody
H-RASIDX antibody
Ha-Ras antibody
HAMSV antibody
HRAS antibody
HRAS1 antibody
K RAS2A antibody
K RAS2B antibody
K RAS4A antibody
K RAS4B antibody
K-RAS antibody
KRAS antibody
KRAS1 antibody
KRAS2 antibody
N-RAS antibody
N-terminally processed antibody
NRAS antibody
NRAS1 antibody
p21ras antibody
RASH_HUMAN antibody
RASH1 antibody
RASK2 antibody
Transforming protein p21 antibody
v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
v ras neuroblastoma RAS viral oncogene homolog antibody
Accession NoSwiss-Prot#:P01111
Uniprot
P01111
Gene ID
4893;
Calculated MW18 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
FC: 1:10-1:100
Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control:
Lane 1: MCF-7
Lane 2: 293T
Lane 3: Mouse brain
Flow cytometric analysis of Hela cells with Ras antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras.
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