Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Recombinant Rabbit Monoclonal Antibodies > Cyclin D1 Rabbit mAb

Cyclin D1 Rabbit mAb#48619

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(1) Other
Product Detail

Product NameCyclin D1 Rabbit mAb

Clone No.SA38-08

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB IHC ICC/IF

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAI327039 antibody
B cell CLL/lymphoma 1 antibody
B cell leukemia 1 antibody
B cell lymphoma 1 protein antibody
B-cell lymphoma 1 protein antibody
BCL 1 antibody
BCL-1 antibody
BCL-1 oncogene antibody
BCL1 antibody
BCL1 oncogene antibody
ccnd1 antibody
CCND1/FSTL3 fusion gene, included antibody
CCND1/IGHG1 fusion gene, included antibody
CCND1/IGLC1 fusion gene, included antibody
CCND1/PTH fusion gene, included antibody
CCND1_HUMAN antibody
cD1 antibody
Cyl 1 antibody
D11S287E antibody
G1/S specific cyclin D1 antibody
G1/S-specific cyclin-D1 antibody
Parathyroid adenomatosis 1 antibody
PRAD1 antibody
PRAD1 oncogene antibody
U21B31 antibody

Accession NoSwiss-Prot#:P24385

Uniprot P24385

Gene ID 595;

Calculated MWPredicted band size: 34 kDa

Sdspage MWObserved band size: 36 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Cyclin D1 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : PC12 whole cell lysates
Lane 3 : SH-SY5Y whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 36 kDa

Exposure time: 4 seconds
All lanes: Cyclin D1 Rabbit mAb at 1/1k dilution

Lane 1 : Rat kidney lysates
Lane 2 : Rat lung lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse lung lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 36 kDa

Exposure time: 8 seconds
All lanes: Cyclin D1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : Cyclin D1 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Cyclin D1 using 48619 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for Cyclin D1 using 48619 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for Cyclin D1 using 48619 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Cyclin D1 antibody (48619)

ICC/IF staining of Cyclin D1 in PC-12 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48619 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Cyclin D1 antibody (48619)

ICC/IF staining of Cyclin D1 in N2A cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48619 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.

If you have published an article using product 48619, please notify us so that we can cite your literature.

et al,Long non-coding RNA TRIM52-AS1 sponges microRNA-577 to facilitate diffuse large B cell lymphoma progression via increasing TRIM52 expression. In Hum Cell on 2022 Jul by Fang Zhao, Shucheng Li,et al..PMID:35676608 , (2022),
PMID: 35676608

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors