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Location： Home > Recombinant Rabbit Monoclonal Antibodies > GAPDH Rabbit mAb

GAPDH Rabbit mAb#48620

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Product Detail

Product NameGAPDH Rabbit mAb

Clone No.1-G2

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt, Monkey, Chicken, zebrafish

Immunogen Descrecombinant protein

ConjugateUnconjugated

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Accession NoSwiss-Prot#:P04406

Uniprot P04406

Gene ID 2597;

Calculated MW36 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:10-1:100
Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/1,000 dilution. Positive control:
Lane 1: NCCIT
Lane 2: PC12
Lane 3: F9
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
ICC staining GAPDH in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GAPDH in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

If you have published an article using product 48620, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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