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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MEK1/2 Rabbit mAb

MEK1/2 Rabbit mAb#48648

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Product Detail

Product NameMEK1/2 Rabbit mAb

Clone No.SR13-07

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu, Ms, Rt, zebrafish

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAA589381 antibody
CFC3 antibody
Dual specificity mitogen-activated protein kinase kinase 1 antibody
Dual specificity mitogen-activated protein kinase kinase 2 antibody
EC 2.7.12.2 antibody
ERK activator kinase 1 antibody
ERK activator kinase 2 antibody
FLJ26075 antibody
MAP kinase kinase 1 antibody
MAP kinase kinase 2 antibody
MAP2K1 antibody
MAP2K2 antibody
MAPK/ERK kinase 1 antibody
MAPK/ERK kinase 2 antibody
MAPKK 1 antibody
MAPKK1 antibody
MAPKK2 antibody
MEK 1 antibody
MEK1 antibody
MEKK1 antibody
Mitogen activated protein kinase kinase 1 antibody
Mitogen activated protein kinase kinase 2 antibody
Mitogen-activated protein kinase kinase 2, p45 antibody
MK2 antibody
MKK 1 antibody
MKK 2 antibody
MKK1 antibody
MKK2 antibody
MP2K1_HUMAN antibody
PRKMK 1 antibody
PRKMK 2 antibody
Prkmk1 antibody
Prkmk2 antibody
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
Protein kinase, mitogen-activated, kinase 1 antibody
Protein kinase, mitogen-activated, kinase 2 antibody

Accession NoSwiss-Prot#:P36507

Uniprot P36507

Gene ID 5605;

Calculated MW44 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of MEK1/2 on different cell lysates using anti-MEK1/2 antibody at 1/1,000 dilution. Positive control:
Lane 1: HepG2
Lane 2: PC12
Lane 3: NIH/3T3
Western blot analysis of MEK1/2 on hybrid fish (crucian-carp) brain tissue lysates using anti-MEK1/2 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
ICC staining MEK1/2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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