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Caspase-3 Rabbit mAb#48658

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Product Detail

Product NameCaspase-3 Rabbit mAb

Clone No.SR03-01

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHuman Mouse

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesA830040C14Rik antibody
Apopain antibody
CASP-3 antibody
CASP3 antibody
CASP3_HUMAN antibody
Casp3a antibody
Caspase 3 antibody
Caspase 3, apoptosis-related cysteine peptidase antibody
Caspase 3, apoptosis-related cysteine protease antibody
Caspase 3, apoptosis-related cysteine protease a antibody
Caspase-3 subunit p12 antibody
CC3 antibody
CPP-32 antibody
CPP32 antibody
CPP32B antibody
Cysteine protease CPP32 antibody
EC 3.4.22.56 antibody
LICE antibody
mldy antibody
OTTHUMP00000165052 antibody
OTTHUMP00000165053 antibody
OTTHUMP00000165054 antibody
PARP cleavage protease antibody
Procaspase3 antibody
Protein Yama antibody
SCA 1 antibody
SCA-1 antibody
SREBP cleavage activity 1 antibody
Yama antibody

Accession NoSwiss-Prot#:P42574

Uniprot P42574

Gene ID 836;

Calculated MW32 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:100

FC:1:20-1:50

Western blot analysis of Caspase-3 on different cell lysates using anti-Caspase-3 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: 293
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
ICC staining Caspase-3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blotting analysis using Caspase-3 Antibody #48658.

Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

If you have published an article using product 48658, please notify us so that we can cite your literature.

et al,Potential role of the cAMP/PKA/CREB signallIng pathway In hypoxic preconditionIng and effect on propofol?Induced neurotoxicity In the hippocampus of neonatal rats.In Mol Med Rep on 2019 Aug by Guan R, Lv J, et al..PMID:31257533 , (2019),
PMID: 31257533

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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