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Location： Home > Recombinant Rabbit Monoclonal Antibodies > beta Tubulin Rabbit mAb

beta Tubulin Rabbit mAb#48659

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Product Detail

Product Namebeta Tubulin Rabbit mAb

Clone No.SR25-04

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesBeta 4 tubulin antibody
Beta 5 tubulin antibody
BetaTubulin antibody
TBB5_HUMAN antibody
TUBB antibody
TUBB2 antibody
TUBB2A antibody
TUBB5 antibody
tubulin beta 2A antibody
Tubulin beta chain antibody
Tubulin beta-5 chain antibody

Accession NoSwiss-Prot#:P07437

Uniprot P07437

Gene ID 203068;

Calculated MW50 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:10-1:100
Western blot analysis of beta Tubulin on different cell lysates using anti-beta Tubulin antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: PC12
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
ICC staining beta Tubulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining beta Tubulin in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining beta Tubulin in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining beta Tubulin in PC12 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining beta Tubulin in SH-SY-5Y cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of NIH/3T3 cells with beta Tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.

If you have published an article using product 48659, please notify us so that we can cite your literature.

et al,DDAH1 Protects against Cardiotoxin-Induced Muscle Injury and Regeneration In Antioxidants (Basel)On2023 Sep 13byFei Feng , Bingqing Cui et al..PMID:37760057 , (2023),
PMID: 37760057
et al,Aerobic Exercise Protects against Cardiotoxin-Induced Skeletal Muscle Injury in a DDAH1-Dependent Manner. In Antioxidants (Basel) on 2024 Sep 1 by Fei Feng, Kai Luo, et al..PMID:39334728 , (2024),
PMID: 39334728

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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