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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Firefly Luciferase Rabbit mAb

Firefly Luciferase Rabbit mAb#48664

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Product Detail

Product NameFirefly Luciferase Rabbit mAb

Clone No.SR03-86

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB,ICC,IF

Species ReactivityFirefly

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Namesec 1.13.12.7 antibody
Firefly antibody
Luciferase antibody
Luciferin 4 monooxygenase antibody

Accession NoSwiss-Prot#:P08659

Uniprot P08659

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000



Western blot analysis of Firefly Luciferase on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

If you have published an article using product 48664, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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