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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Renilla Luciferase Rabbit mAb

Renilla Luciferase Rabbit mAb#48665

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Product Detail

Product NameRenilla Luciferase Rabbit mAb

Clone No.SR07-830

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB,ICC,IF

Species ReactivitySea pansy

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesRenilla luciferin 2 monooxygenase antibody
Renilla type luciferase antibody

Accession NoSwiss-Prot#:P27652

Uniprot P27652

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000



Western blot analysis of Renilla Luciferase on luciferase recombinant protein lysate using anti-Renilla Luciferase antibody at 1/1,000 dilution.
Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

If you have published an article using product 48665, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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