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Cdk2 Rabbit mAb#48670

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Product Detail

Product NameCdk2 Rabbit mAb

Clone No.SR01-01

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCdc2 related protein kinase antibody
cdc2-related protein kinase antibody
CDC28 antibody
CDC2A antibody
Cdk 2 antibody
CDK1 antibody
CDK2 antibody
CDK2_HUMAN antibody
CDKN2 antibody
Cell devision kinase 2 antibody
Cell division protein kinase 2 antibody
Cyclin dependent kinase 2 antibody
cyclin dependent kinase 2-alpha antibody
Cyclin-dependent kinase 2 antibody
kinase Cdc2 antibody
MPF antibody
p33 protein kinase antibody
p33(CDK2) antibody

Accession NoSwiss-Prot#:P24941

Uniprot P24941

Gene ID 1017;

Calculated MW33 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Cdk2 on different cell lysates using anti-Cdk2 antibody at 1/1,000 dilution. Positive control:
Lane 1: A549
Lane 2: F9
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
ICC staining Cdk2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in SH-SY-5Y cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. It acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1.

If you have published an article using product 48670, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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