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Location： Home > Recombinant Rabbit Monoclonal Antibodies > HP1 alpha Rabbit mAb

HP1 alpha Rabbit mAb#48672

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Product Detail

Product NameHP1 alpha Rabbit mAb

Clone No.SR44-03

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAntigen p25 antibody
CBX5 antibody
CBX5_HUMAN antibody
CG8409 antibody
Chromobox 5 antibody
Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody
Chromobox homolog 5 antibody
Chromobox protein homolog 5 antibody
Epididymis luminal protein 25 antibody
HEL25 antibody
Heterochromatin protein 1 alpha antibody
Heterochromatin protein 1 antibody
Heterochromatin protein 1 homolog alpha antibody
HP1 alpha antibody
HP1 alpha homolog antibody
HP1 antibody
HP1A antibody
HP1Hs alpha antibody
Su(var)205 antibody

Accession NoSwiss-Prot#:P45973

Uniprot P45973

Gene ID 23468;

Calculated MW22 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of HP1 alpha on different cell lysates using anti-HP1 alpha antibody at 1/1,000 dilution. Positive control:
Lane 1: MCF-7
Lane 2: Hela
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
ICC staining HP1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining HP1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5Y cells with HP1 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The family of Heterochromatin Protein 1 (HP1) ("Chromobox Homolog", CBX) are highly conserved adapter proteins, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.Three different paralogs of HP1 are found in mammals, HP1α, HP1βand HP1γ.

If you have published an article using product 48672, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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