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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MEK1 Rabbit mAb

MEK1 Rabbit mAb#48682

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Product Detail

Product NameMEK1 Rabbit mAb

Clone No.SZ22-01

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt, Cow, Dog

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesDual specificity mitogen activated protein kinase kinase 1 antibody
Dual specificity mitogen-activated protein kinase kinase 1 antibody
ERK activator kinase 1 antibody
MAP kinase kinase 1 antibody
MAP kinase/Erk kinase 1 antibody
MAP2K1 antibody
MAPK/ERK kinase 1 antibody
MAPKK 1 antibody
MAPKK1 antibody
MEK 1 antibody
Mek1 antibody
MEKK1 antibody
Mitogen activated protein kinase kinase 1 antibody
MKK 1 antibody
MKK1 antibody
MP2K1_HUMAN antibody
PRKMK1 antibody
Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
Protein kinase mitogen activated, kinase 1 antibody

Accession NoSwiss-Prot#:Q02750

Uniprot Q02750

Gene ID 5604;

Calculated MW43 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of MEK1 on different lysates using anti-MEK1 antibody at 1/1,000 dilution. Positive control:
Lane 1: A431
Lane 2: HepG2
Lane 3: Hela
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-MEK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MEK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEK1 antibody. Counter stained with hematoxylin.
ICC staining MEK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MEK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MEK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with MEK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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