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Location： Home > Recombinant Rabbit Monoclonal Antibodies > ERK2 Rabbit mAb

ERK2 Rabbit mAb#48684

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Product Detail

Product NameERK2 Rabbit mAb

Clone No.SZ25-01

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesERK 2 antibody
ERK antibody
ERK-2 antibody
ERT1 antibody
Extracellular Signal Regulated Kinase 2 antibody
Extracellular signal-regulated kinase 2 antibody
MAP kinase 1 antibody
MAP kinase 2 antibody
MAP kinase isoform p42 antibody
MAPK 1 antibody
MAPK 2 antibody
Mapk1 antibody
MAPK2 antibody
Mitogen-activated protein kinase 1 antibody
Mitogen-activated protein kinase 2 antibody
MK01_HUMAN antibody
P38 antibody
P40 antibody
P41 antibody
p42-MAPK antibody
P42MAPK antibody
PRKM1 antibody
PRKM2 antibody
protein kinase, mitogen-activated, 1 antibody
protein kinase, mitogen-activated, 2 antibody
protein tyrosine kinase ERK2 antibody

Accession NoSwiss-Prot#:P28482

Uniprot P28482

Gene ID 5594;

Calculated MW41 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
ICC staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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