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AIF Rabbit mAb#48692

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Product Detail

Product NameAIF Rabbit mAb

Clone No.SZ05-01

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAIFM1 antibody
AIFM1_HUMAN antibody
Apoptosis inducing factor 1, mitochondrial antibody
Apoptosis inducing factor antibody
Apoptosis inducing factor, mitochondrion associated, 1 antibody
Apoptosis-inducing factor 1 antibody
CMTX4 antibody
COWCK antibody
COXPD6 antibody
Harlequin antibody
Hq antibody
mAIF antibody
MGC111425 antibody
MGC5706 antibody
mitochondrial antibody
Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody
neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody
PDCD 8 antibody
PDCD8 antibody
Programmed cell death 8 (apoptosis inducing factor) antibody
Programmed cell death 8 antibody
Programmed cell death 8 isoform 1 antibody
Programmed cell death 8 isoform 2 antibody
Programmed cell death 8 isoform 3 antibody
Programmed cell death protein 8 antibody
Programmed cell death protein 8 mitochondrial antibody
Programmed cell death protein 8 mitochondrial precursor antibody
Programmed cell death protein 8 mitochondrial precursor antibody
Striatal apoptosis inducing factor antibody

Accession NoSwiss-Prot#:O95831

Uniprot O95831

Gene ID 9131;

Calculated MW67 kDa

Sdspage MW67 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AIF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AIF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-AIF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AIF antibody. Counter stained with hematoxylin.
ICC staining AIF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AIF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with AIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes : AIF Rabbit mAb at 1/1k dilution

Lane 1 : SKOV-3 whole cell lysates
Lane 2 : Hela whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : C6 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 7 seconds
All lanes : AIF Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : AIF knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.

If you have published an article using product 48692, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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