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ZO1 Polyclonal Antibody#48694

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Product Detail

Product NameZO1 Polyclonal Antibody

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB;IF;ELISA

Species ReactivityHuman;Mouse;Pig

SpecificityZO1 Polyclonal Antibody detects endogenous levels of protein.

Immunogen DescSynthesized peptide derived from part region of human protein.AA1600-1700

Target NameTJP1 ZO1

ConjugateUnconjugated

Uniprot Q07157

Gene ID 7082

Calculated MW192kD

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, and 0.02% sodium azide.

StorageStore at -20℃/1 year

Application Details

 WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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