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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Cytokeratin 18 Rabbit mAb

Cytokeratin 18 Rabbit mAb#48696

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Product Detail

Product NameCytokeratin 18 Rabbit mAb

Clone No.SZ80-07

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCell proliferation inducing gene 46 protein antibody
Cell proliferation inducing protein 46 antibody
Cell proliferation-inducing gene 46 protein antibody
CK 18 antibody
CK-18 antibody
CK18 antibody
CYK 18 antibody
CYK18 antibody
Cytokeratin 18 antibody
Cytokeratin endo B antibody
Cytokeratin-18 antibody
K 18 antibody
K18 antibody
K1C18_HUMAN antibody
KA18 antibody
Keratin 18 antibody
Keratin 18, type I antibody
Keratin D antibody
keratin, type I cytoskeletal 18 antibody
Keratin-18 antibody
Krt18 antibody

Accession NoSwiss-Prot#:P05783

Uniprot P05783

Gene ID 3875;

Calculated MW48 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:10,000-1:50,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of Cytokeratin 18 on different lysates using anti-Cytokeratin 18 antibody at 1/20,000 dilution. Positive control:
Lane 1: A431
Lane 2: Mouse colon
Lane 3: Mouse kidney
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.
ICC staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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