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Location： Home > Recombinant Rabbit Monoclonal Antibodies > FOXO3A Rabbit mAb

FOXO3A Rabbit mAb#48698

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Product Detail

Product NameFOXO3A Rabbit mAb

Clone No.SP0054

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAF6q21 antibody
AF6q21 protein antibody
DKFZp781A0677 antibody
FKHR2 antibody
FKHRL 1 antibody
FKHRL1 antibody
FKHRL1P2 antibody
Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
Forkhead box O3 antibody
Forkhead box O3A antibody
Forkhead box protein O3 antibody
Forkhead box protein O3A antibody
Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
Forkhead homolog (rhabdomyosarcoma) like 1 antibody
Forkhead in rhabdomyosarcoma like 1 antibody
Forkhead in rhabdomyosarcoma-like 1 antibody
FOX O3A antibody
FOXO2 antibody
foxo3 antibody
FOXO3_HUMAN antibody
FOXO3A antibody
MGC12739 antibody
MGC31925 antibody

Accession NoSwiss-Prot#:O43524

Uniprot O43524

Gene ID 2309;

Calculated MW71 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000

ICC: 1:50-1:200

Western blot analysis of FOXO3A on different lysates using anti- FOXO3A antibody at 1/1,000 dilution. Positive control:
Lane 1: NIH/3T3
Lane 2: PC-12
Lane 3: MCF-7
ICC staining FOXO3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining FOXO3A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining FOXO3A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
FKHRL1 (forkhead in rhabdomyosarcoma-like 1), also known as FOXO3 (forkheadbox O3) or FOXO3A, is a 673 amino acid transcriptional activator thatbelongs to the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at Threonine 32 and Serine 253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephosphorylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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