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Tubulin beta-III Rabbit mAb#48701

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Product Detail

Product NameTubulin beta-III Rabbit mAb

Clone No.SP06-00

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namesbeta 3 tubulin antibody
beta-4 antibody
CDCBM antibody
CDCBM1 antibody
CFEOM3 antibody
CFEOM3A antibody
FEOM3 antibody
M(beta)3 antibody
M(beta)6 antibody
MC1R antibody
Neuron specific beta III Tubulin antibody
Neuron-specific class III beta-tubulin antibody
QccE-11995 antibody
QccE-15186 antibody
TBB3_HUMAN antibody
Tubb 3 antibody
TUBB3 antibody
TUBB4 antibody
Tubulin beta 3 antibody
Tubulin beta 3 chain antibody
Tubulin beta 4 antibody
Tubulin beta III antibody
Tubulin beta-3 chain antibody
Tubulin beta-4 chain antibody
Tubulin beta-III antibody

Accession NoSwiss-Prot#:Q13509

Uniprot Q13509

Gene ID 10381;

Calculated MW50 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of Tubulin beta-III on different lysates using anti-Tubulin beta-III antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: SH-SY-5Y
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tubulin beta-III antibody. Counter stained with hematoxylin.
Flow cytometric analysis of N2A cells with Tubulin beta-III antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

If you have published an article using product 48701, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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