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Smad2 Rabbit mAb#48703

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Product Detail

Product NameSmad2 Rabbit mAb

Clone No.SP06-05

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesDrosophila, homolog of, MADR2 antibody
hMAD-2 antibody
HsMAD2 antibody
JV18 antibody
JV18-1 antibody
JV181 antibody
MAD antibody
MAD homolog 2 antibody
MAD Related Protein 2 antibody
Mad-related protein 2 antibody
MADH2 antibody
MADR2 antibody
MGC22139 antibody
MGC34440 antibody
Mother against DPP homolog 2 antibody
Mothers against decapentaplegic homolog 2 antibody
Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody
Mothers against DPP homolog 2 antibody
OTTHUMP00000163489 antibody
Sma and Mad related protein 2 antibody
Sma- and Mad-related protein 2 MAD antibody
SMAD 2 antibody
SMAD family member 2 antibody
SMAD, mothers against DPP homolog 2 antibody
SMAD2 antibody
SMAD2_HUMAN antibody

Accession NoSwiss-Prot#:Q15796

Uniprot Q15796

Gene ID 4087;

Calculated MW58 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Smad2 on different lysates using anti-Smad2 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: A431
Lane 3: PC12
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Smad2 antibody. Counter stained with hematoxylin.

ICC staining Smad2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of NIH/3T3 cells with Smad2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

If you have published an article using product 48703, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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