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Location： Home > Recombinant Rabbit Monoclonal Antibodies > ERK1 Rabbit mAb

ERK1 Rabbit mAb#48709

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Product Detail

Product NameERK1 Rabbit mAb

Clone No.SP05-09

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesERK 1 antibody
ERK antibody
ERK-1 antibody
ERK1 antibody
ERT 2 antibody
ERT2 antibody
Extracellular Signal Regulated Kinase 1 antibody
Extracellular signal related kinase 1 antibody
Extracellular signal-regulated kinase 1 antibody
HGNC6877 antibody
HS44KDAP antibody
HUMKER1A antibody
Insulin Stimulated MAP2 Kinase antibody
Insulin-stimulated MAP2 kinase antibody
MAP kinase 1 antibody
MAP kinase 3 antibody
MAP Kinase antibody
MAP kinase isoform p44 antibody
MAPK 1 antibody
MAPK 3 antibody
MAPK antibody
MAPK1 antibody
Mapk3 antibody
MGC20180 antibody
Microtubule Associated Protein 2 Kinase antibody
Microtubule-associated protein 2 kinase antibody
Mitogen Activated Protein Kinase 3 antibody
Mitogen-activated protein kinase 1 antibody
Mitogen-activated protein kinase 3 antibody
MK03_HUMAN antibody
OTTHUMP00000174538 antibody
OTTHUMP00000174541 antibody
p44 ERK1 antibody
p44 MAPK antibody
p44-ERK1 antibody
p44-MAPK antibody
P44ERK1 antibody
P44MAPK antibody
PRKM 3 antibody
PRKM3 antibody
Protein Kinase Mitogen Activated 3 antibody

Accession NoSwiss-Prot#:P27361

Uniprot P27361

Gene ID 5595;

Calculated MW43 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: K562
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p12-p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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