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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Heme Oxygenase 1(HO-1) Rabbit mAb

Heme Oxygenase 1(HO-1) Rabbit mAb#48715

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Product Detail

Product NameHeme Oxygenase 1(HO-1) Rabbit mAb

Clone No.SP08-07

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, IP, FC

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names32 kD antibody
bK286B10 antibody
D8Wsu38e antibody
heat shock protein 32 kD antibody
heat shock protein 32kD antibody
Heat shock protein antibody
Heme oxygenase (decycling) 1 antibody
Heme oxygenase 1 antibody
Hemox antibody
HMOX 1 antibody
Hmox antibody
Hmox1 antibody
HMOX1_HUMAN antibody
HO 1 antibody
HO antibody
HO-1 antibody
HO1 antibody
Hsp32 antibody

Accession NoSwiss-Prot#:P09601

Uniprot P09601

Gene ID 3162;

Calculated MW33 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC:1:50-1:200


FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HO-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Jurkat cells with HO-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.
Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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