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VEGF Receptor 1 Rabbit mAb#48718

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Product Detail

Product NameVEGF Receptor 1 Rabbit mAb

Clone No.SY09-09

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesEC 2.7.10.1 antibody
FLT 1 antibody
FLT antibody
Flt-1 antibody
FLT1 antibody
Fms like tyrosine kinase 1 antibody
Fms related tyrosine kinase 1 antibody
Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
Fms-like tyrosine kinase 1 antibody
FRT antibody
Soluble VEGF receptor 1 14 antibody
Soluble VEGFR1 variant 2 antibody
Soluble VEGFR1 variant 21 antibody
Tyrosine protein kinase FRT antibody
Tyrosine protein kinase receptor FLT antibody
Tyrosine-protein kinase FRT antibody
Tyrosine-protein kinase receptor FLT antibody
Vascular endothelial growth factor receptor 1 antibody
Vascular endothelial growth factor vascular permeability factor receptor antibody
Vascular permeability factor receptor 1 antibody
Vascular permeability factor receptor antibody
VEGFR 1 antibody
VEGFR-1 antibody
VEGFR1 antibody
VGFR1_HUMAN antibody

Accession NoSwiss-Prot#:P17948

Uniprot P17948

Gene ID 2321;

Calculated MW151 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of VEGF Receptor 1 on mouse lung lysates using anti-VEGF Receptor 1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.
ICC staining VEGF Receptor 1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF Receptor 1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF Receptor 1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A431 cells with VEGF Receptor 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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