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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PCNA Rabbit mAb

PCNA Rabbit mAb#48728

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Product Detail

Product NamePCNA Rabbit mAb

Clone No.SY12-07

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesATLD2 antibody
cb16 antibody
Cyclin antibody
DNA polymerase delta auxiliary protein antibody
etID36690.10 antibody
fa28e03 antibody
fb36g03 antibody
HGCN8729 antibody
MGC8367 antibody
Mutagen-sensitive 209 protein antibody
OTTHUMP00000030189 antibody
OTTHUMP00000030190 antibody
PCNA antibody
Pcna/cyclin antibody
PCNA_HUMAN antibody
PCNAR antibody
Polymerase delta accessory protein antibody
Proliferating cell nuclear antigen antibody
wu:fa28e03 antibody
wu:fb36g03 antibody

Accession NoSwiss-Prot#:P12004

Uniprot P12004

Gene ID 5111;

Calculated MW29 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:100-1:500
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: 293
Lane 3: A431
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.

If you have published an article using product 48728, please notify us so that we can cite your literature.

et al,Based on network pharmacology, gastrodin attenuates hypertension-induced vascular smooth muscle cell proliferation and PI3K/AKT pathway activationInSci RepOn 2023 Jul 26byAling Shen , Meizhu Wu et al..PMID:37495624 , (2023),
PMID: 37495624

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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