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Hsp90 beta Rabbit mAb#48736

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Product Detail

Product NameHsp90 beta Rabbit mAb

Clone No.SY46-01

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names90 kda heat shock protein beta HSP90 beta antibody
D6S182 antibody
FLJ26984 antibody
Heat shock 84 kDa antibody
Heat shock 90kD protein 1, beta antibody
Heat shock 90kDa protein 1 beta antibody
Heat shock protein 90 alpha family class B member 1 antibody
Heat shock protein 90 kDa antibody
Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
Heat shock protein 90kDa alpha family class B member 1 antibody
Heat shock protein beta antibody
Heat shock protein HSP 90 beta antibody
Heat shock protein HSP 90-beta antibody
HS90B_HUMAN antibody
HSP 84 antibody
HSP 90 antibody
HSP 90 b antibody
HSP 90b antibody
HSP84 antibody
HSP90 BETA antibody
hsp90ab1 antibody
HSP90B antibody
HSPC2 antibody
HSPCB antibody

Accession NoSwiss-Prot#:P08238

Uniprot P08238

Gene ID 3326;

Calculated MW83 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of Hsp90 beta on different lysates using anti-Hsp90 beta antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: K562
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.
ICC staining Hsp90 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Hsp90 beta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Hsp90 beta in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with Hsp90 beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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