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Hsp90 alpha Rabbit mAb#48737

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Product Detail

Product NameHsp90 alpha Rabbit mAb

Clone No.SY14-06

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesEL52 antibody
epididymis luminal secretory protein 52 antibody
Heat shock 86 kDa antibody
heat shock 90kD protein 1, alpha antibody
Heat shock 90kD protein 1, alpha like 4 antibody
heat shock 90kD protein, alpha-like 4 antibody
Heat shock 90kDa protein 1 alpha antibody
Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
Heat shock protein HSP 90-alpha antibody
HS90A_HUMAN antibody
HSP 86 antibody
HSP86 antibody
Hsp89 antibody
HSP89A antibody
Hsp90 antibody
HSP90A antibody
HSP90AA1 antibody
HSP90ALPHA antibody
HSP90N antibody
HSPC1 antibody
HSPCA antibody
HSPCAL1 antibody
HSPCAL4 antibody
HSPN antibody
LAP 2 antibody
LAP2 antibody
lipopolysaccharide-associated protein 2 antibody
LPS-associated protein 2 antibody
Renal carcinoma antigen NY-REN-38 antibody

Accession NoSwiss-Prot#:P07900

Uniprot P07900

Gene ID 3320;

Calculated MW90 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Hsp90 alpha on COS-1 cell lysates using anti-Hsp90 alpha antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp90 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hsp90 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Hsp90 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Hsp90 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Hsp90 alpha antibody. Counter stained with hematoxylin.
ICC staining Hsp90 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Hsp90 alpha in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Hsp90 alpha in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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