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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MCL1 Rabbit mAb

MCL1 Rabbit mAb#48741

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Product Detail

Product NameMCL1 Rabbit mAb

Clone No.SI16-04

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesBcl 2 related protein EAT/mcl1 antibody
Bcl-2-like protein 3 antibody
Bcl-2-related protein EAT/mcl1 antibody
BCL2 related antibody
Bcl2-L-3 antibody
BCL2L3 antibody
EAT antibody
Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
MCL 1 antibody
MCL1 antibody
MCL1-ES antibody
mcl1/EAT antibody
MCL1_HUMAN antibody
MCL1L antibody
MCL1S antibody
MGC104264 antibody
MGC1839 antibody
Myeloid Cell Leukemia 1 antibody
Myeloid cell leukemia ES antibody
Myeloid cell leukemia sequence 1 antibody
Myeloid cell leukemia sequence 1 BCL2 related antibody
Myeloid cell leukemia sequence 1 isoform 1 antibody
OTTHUMP00000032794 antibody
OTTHUMP00000032795 antibody
TM antibody

Accession NoSwiss-Prot#:Q07820

Uniprot Q07820

Gene ID 4170;

Calculated MW37/29 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of MCL1 on different lysates using anti-MCL1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Raji
Lane 2: Mouse heart
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MCL1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MCL1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MCL1 antibody. Counter stained with hematoxylin.
ICC staining MCL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MCL1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MCL1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with MCL1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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