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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Smad5 Rabbit mAb

Smad5 Rabbit mAb#48746

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Product Detail

Product NameSmad5 Rabbit mAb

Clone No.SI17-06

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesDKFZp781C1895 antibody
DKFZp781O1323 antibody
Dwfc antibody
hSmad5 antibody
JV5 1 antibody
JV5-1 antibody
MAD homolog 5 antibody
MAD, mothers against decapentaplegic homolog 5 antibody
MADH 5 antibody
MADH5 antibody
Mothers against decapentaplegic homolog 5 antibody
mothers against decapentaplegic, drosophila, homolog of, 5 antibody
Mothers against DPP homolog 5 antibody
MusMLP antibody
SMA and MAD related protein 5 antibody
SMAD 5 antibody
SMAD family member 5 antibody
SMAD, mothers against DPP homolog 5 antibody
Smad5 antibody
SMAD5_HUMAN antibody

Accession NoSwiss-Prot#:Q99717

Uniprot Q99717

Gene ID 4090;

Calculated MW52 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Smad5 on PC-3M cell lysates using anti-Smad5 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Smad5 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Smad5 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Smad5 antibody. Counter stained with hematoxylin.
ICC staining Smad5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad5 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad5 in PC-3M cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Smad5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

If you have published an article using product 48746, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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