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Desmin Rabbit mAb#48749

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Product Detail

Product NameDesmin Rabbit mAb

Clone No.SI18-00

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt, zebrafish

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCMD1I antibody
CSM1 antibody
CSM2 antibody
DES antibody
DESM_HUMAN antibody
Desmin antibody
FLJ12025 antibody
FLJ39719 antibody
FLJ41013 antibody
FLJ41793 antibody
Intermediate filament protein antibody
OTTHUMP00000064865 antibody

Accession NoSwiss-Prot#:P17661

Uniprot P17661

Gene ID 1674;

Calculated MW53 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Desmin on different lysates using anti-Desmin antibody at 1/1,000 dilution. Positive control:
Lane 1: Human skeletal muscle
Lane 2: Mouse heart
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Desmin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Desmin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Desmin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Desmin antibody. Counter stained with hematoxylin.
ICC staining Desmin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of C2C12 cells with Desmin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Desmin makes up attachments between the terminal Z-disc and membrane-associated proteins to form a force-transmitting system. Mutations in the gene encoding for Desmin are associated with adult-onset skeletal myopathy, sporadic disease and mild cardiac involvement.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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