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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Met(C-Met) Rabbit mAb

Met(C-Met) Rabbit mAb#48758

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Product Detail

Product NameMet(C-Met) Rabbit mAb

Clone No.SJ19-05

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAUTS9 antibody
c met antibody
D249 antibody
Hepatocyte growth factor receptor antibody
HGF antibody
HGF receptor antibody
HGF/SF receptor antibody
HGFR antibody
MET antibody
Met proto oncogene tyrosine kinase antibody
MET proto oncogene, receptor tyrosine kinase antibody
Met proto-oncogene (hepatocyte growth factor receptor) antibody
Met proto-oncogene antibody
Met protooncogene antibody
MET_HUMAN antibody
Oncogene MET antibody
Par4 antibody
Proto-oncogene c-Met antibody
RCCP2 antibody
Scatter factor receptor antibody
SF receptor antibody
Tyrosine-protein kinase Met antibody

Accession NoSwiss-Prot#:P08581

Uniprot P08581

Gene ID 4233;

Calculated MW155 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Met on different lysates using anti-Met antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: HepG2
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Met antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Met antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Met antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Met antibody. Counter stained with hematoxylin.
ICC staining Met in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Met antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines . The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

If you have published an article using product 48758, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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