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MMP14 Rabbit mAb#48761

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Product Detail

Product NameMMP14 Rabbit mAb

Clone No.3-F7

Clonality Monoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen DescA synthesized peptide derived from human MMP14

ConjugateUnconjugated

Other NamesMatrix metallopeptidase 14 (membrane inserted) antibody
Matrix metalloproteinase 14 antibody
Matrix metalloproteinase-14 antibody
Membrane type 1 matrix metalloproteinase antibody
Membrane type 1 metalloprotease antibody
Membrane type matrix metalloproteinase 1 antibody
Membrane-type matrix metalloproteinase 1 antibody
Membrane-type-1 matrix metalloproteinase antibody
MMP 14 antibody
MMP X1 antibody
MMP-14 antibody
MMP-X1 antibody
Mmp14 antibody
MMP14_HUMAN antibody
MMPX1 antibody
MT MMP 1 antibody
MT-MMP 1 antibody
MT1 MMP antibody
MT1-MMP antibody
MT1MMP antibody
MTMMP 1 antibody
MTMMP1 antibody

Accession NoSwiss-Prot#:P50281

Uniprot P50281

Gene ID 4323;

Calculated MW65 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

WB 1:1000-1:2000;
IHC 1:100-1:200;
ICC/IF 1:50-1:200;
IP 1:20-1:50;
FC 1:20-1:100

Western blot analysis of MMP14 on human kidney tissue lysates using anti-MMP14 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
ICC staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MMP14 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover.

If you have published an article using product 48761, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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