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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Integrin beta 3 Rabbit mAb

Integrin beta 3 Rabbit mAb#48762

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Product Detail

Product NameIntegrin beta 3 Rabbit mAb

Clone No.SJ19-09

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHu, Ms, Rt

Immunogen DescA synthesized peptide derived from human Integrin beta 3

ConjugateUnconjugated

Other NamesBDPLT16 antibody
BDPLT2 antibody
CD 61 antibody
CD61 antibody
CD61 antigen antibody
GP3A antibody
GPIIIa antibody
GT antibody
HPA 1 antibody
HPA 4 antibody
Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
Integrin beta chain beta 3 antibody
Integrin beta-3 antibody
ITB3_HUMAN antibody
ITG B3 antibody
ITGB 3 antibody
ITGB3 antibody
NAIT antibody
Platelet fibrinogen receptor beta subunit antibody
Platelet fibrinogen receptor, beta subunit antibody
Platelet glycoprotein IIIa antibody
Platelet glycoprotein IIIa precursor antibody
Platelet membrane glycoprotein IIIa antibody
PTP antibody

Accession NoSwiss-Prot#:P05106

Uniprot P05106

Gene ID 3690;

Calculated MWPredicted band size: 87 kDa

Sdspage MWObserved band size: 100 kDa

Concentration0.4mg/ml

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Integrin beta 3 Rabbit mAb at 1/1k dilution

Lane 1 : HUVEC whole cell lysates
Lane 2 : U-87 MG whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 100 kDa

Exposure time: 8 seconds
All lanes: Integrin beta 3 Rabbit mAb at 1/1k dilution

Lane 1 : Mouse stomach lysates
Lane 2 : Rat colon lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 100 kDa

Exposure time: 11 seconds
All lanes: Integrin beta 3 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 : Integrin beta 3 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Integrin beta 3 using 48762 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Integrin beta 3 using 48762 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse testis tissue stained for Integrin beta 3 using 48762 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Integrin beta 3 antibody (48762)

ICC/IF staining of Integrin beta 3 in HepG2 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48762 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control.
Nuclei were counterstained with DAPI.

Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

If you have published an article using product 48762, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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