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Location： Home > Recombinant Rabbit Monoclonal Antibodies > CD86 Rabbit mAb

CD86 Rabbit mAb#48763

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Product Detail

Product NameCD86 Rabbit mAb

Clone No.SJ20-00

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Rt

Immunogen DescSynthetic peptide within Human CD86 aa 1-50 / 329.

ConjugateUnconjugated

Other NamesActivation B7-2 antigen 3 antibody
Activation B7-2 antigen antibody
B-lymphocyte activation antigen B7-2 2 antibody
B-lymphocyte activation antigen B7-2 antibody
B7 2 antibody
B7 antibody
B7-2 antibody
B7.2 antibody
B70 antibody
B72 antigen antibody
BU63 antibody
CD28 antigen ligand 2 2 antibody
CD28 antigen ligand 2 antibody
Cd28l2 antibody
CD28LG2 antibody
CD86 antibody
CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
CD86 antigen antibody
CD86 molecule antibody
CD86_HUMAN antibody
CLS1 antibody
CTLA-4 counter-receptor B7.2 2, 3 antibody
CTLA-4 counter-receptor B7.2 antibody
Early T-cell costimulatory molecule 1 antibody
ETC-1 antibody
FUN 1 antibody
FUN-1 antibody
LAB72 antibody
Ly-58 antibody
Ly58 antibody
MB7 antibody
MB7-2 antibody
Membrane glycoprotein antibody
MGC34413 antibody
T-lymphocyte activation antigen CD86 antibody
TS/A-2 antibody

Accession NoSwiss-Prot#:P42081

Uniprot P42081

Gene ID 942;

Calculated MW38 kDa

Concentration1 mg/ml

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
IP: Use at an assay dependent concentration.

Western blot analysis of CD86 on different lysates using anti-CD86 antibody at 1/1,000 dilution. Positive control:
Lane 1: Raji
Lane 2: Jurkat
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD86 antibody. Counter stained with hematoxylin.
ICC staining CD86 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD86 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD86 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins B7-1 (CD80) and B7-2 (CD86) expressed on antigen presenting cells bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28, and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.

If you have published an article using product 48763, please notify us so that we can cite your literature.

et al,Human Umbilical Cord Mesenchymal Stem Cells Improve the Necrosis and Osteocyte Apoptosis in Glucocorticoid-Induced Osteonecrosis of the Femoral Head Model through Reducing the Macrophage Polarization. In Int J Stem Cells on 2021 Dec 31 by Gang Tian, Chuanjie Liu,al..PMID:34965999 , (2021),
PMID: 34965999
et al,Preliminary Study on the Antigen-Removal from Extracellular Matrix via Different Decellularization. In Tissue Eng Part C Methods on 2022 Jun by Huan Wu, Guangfu Yin, et al..PMID: 35596569 , (2022),
PMID: 35596569
et al,Human Umbilical Cord Mesenchymal Stem Cells Improve the Necrosis and Osteocyte Apoptosis in Glucocorticoid-Induced Osteonecrosis of the Femoral Head Model through Reducing the Macrophage Polarization. In Int J Stem Cells on 2022 May 30 by Gang Tian, Chuanjie Liu, et al..PMID:34965999 , (2022),
PMID: 34965999

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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