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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Notch 1 Rabbit mAb

Notch 1 Rabbit mAb#48766

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Product Detail

Product NameNotch 1 Rabbit mAb

Clone No.SJ205

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names9930111A19Rik antibody
AOS5 antibody
AOVD1 antibody
hN1 antibody
Lin-12 antibody
LIN12 antibody
Mis6 antibody
Motch A antibody
mT14 antibody
Neurogenic locus notch homolog protein 1 antibody
Neurogenic locus notch protein homolog antibody
NICD antibody
NOTC1_HUMAN antibody
Notch 1 antibody
Notch 1 intracellular domain antibody
NOTCH antibody
Notch gene homolog 1 (Drosophila) antibody
Notch homolog 1, translocation-associated (Drosophila) antibody
NOTCH, Drosophila, homolog of, 1 antibody
notch1 antibody
TAN1 antibody
Translocation associated notch homolog antibody
Translocation associated notch protein TAN 1 antibody
Translocation-associated notch protein TAN-1 antibody
xotch antibody

Accession NoSwiss-Prot#:P46531

Uniprot P46531

Gene ID 4851;

Calculated MW125 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Notch 1 on HepG2 cell lysates using anti-Notch 1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.
ICC staining Notch 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Notch 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Notch 1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Notch 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The LIN-12/notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. To date, four notch homologs have been identified in mammals and have been designated Notch 1, Notch 2, Notch 3 and Notch 4. The notch genes are expressed in a variety of tissues in both the embryonic and adult organism, suggesting that the genes are involved in multiple signaling pathways. The notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for notch include Jagged1, Jagged2 and Delta. Jagged can activate notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 is thought to be involved in the development of various tissues whose development is dependent upon epithelial-mesenchymal interactions. Normal Delta expression is restricted to the adrenal gland and placenta. Delta expression has also been found in neuroendocrine tumors such as neuroblastomas and pheochromocytomas.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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