Product Detail
Product NameCDC7 Kinase Rabbit mAb
Clone No.SY21-01
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesCdc 7 antibody
CDC7 antibody
CDC7 cell division cycle 7 antibody
CDC7 cell division cycle 7 like 1 antibody
CDC7 L1 antibody
Cdc7 like 1 antibody
CDC7 related kinase antibody
CDC7-related kinase antibody
CDC7_HUMAN antibody
CDC7L 1 antibody
CDC7L1 antibody
Cell division cycle 7 homolog antibody
Cell division cycle 7 kinase antibody
Cell division cycle 7 like protein 1 antibody
Cell division cycle 7 related protein kinase antibody
Cell division cycle 7-related protein kinase antibody
HsCDC 7 antibody
HsCdc7 antibody
Hsk 1 antibody
Hsk1 antibody
HuCDC 7 antibody
huCdc7 antibody
MGC117361 antibody
MGC126237 antibody
MGC126238 antibody
Accession NoSwiss-Prot#:O00311
Uniprot
O00311
Gene ID
8317;
Calculated MW64 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
FC: 1:50-1:100
Western blot analysis of CDC7 Kinase on different lysates using anti-CDC7 antibody at 1/1,000 dilution. Positive control:
Lane 1: Jurkat
Lane 2: Hela
Flow cytometric analysis of A431 cells with CDC7 Kinase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7/Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase a-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4. The kinase activity of the complex is regulated throughout the cell cycle, mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.
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