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Paxillin Rabbit mAb#48774

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Product Detail

Product NamePaxillin Rabbit mAb

Clone No.SY23-02

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesFLJ16691 antibody
FLJ23042 antibody
Paired box protein Pax 1 antibody
PAX 1 antibody
PAX1 antibody
PAXI_HUMAN antibody
Paxillin alpha antibody
Paxillin antibody
PXN antibody
PXN protein antibody

Accession NoSwiss-Prot#:P49023

Uniprot P49023

Gene ID 5829;

Calculated MW65 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Paxillin on NIH/3T3 cells lysates using anti-Paxillin antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kideny tissue using anti-Paxillin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Paxillin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Paxillin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Paxillin antibody. Counter stained with hematoxylin.
ICC staining Paxillin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Paxillin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Paxillin in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-Src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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