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Location： Home > Recombinant Rabbit Monoclonal Antibodies > N Cadherin Rabbit mAb

N Cadherin Rabbit mAb#48779

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Product Detail

Product NameN Cadherin Rabbit mAb

Clone No.SY02-46

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCADH2_HUMAN antibody
Cadherin 2 antibody
Cadherin 2 N cadherin neuronal antibody
Cadherin 2 type 1 antibody
Cadherin 2 type 1 N cadherin neuronal antibody
Cadherin 2, type 1, N-cadherin (neuronal) antibody
Cadherin-2 antibody
Cadherin2 antibody
Calcium dependent adhesion protein neuronal antibody
CD325 antibody
CD325 antigen antibody
CDH2 antibody
CDHN antibody
CDw325 antibody
CDw325 antigen antibody
N cadherin 1 antibody
N-cadherin antibody
NCAD antibody
Neural cadherin antibody
OTTHUMP00000066304 antibody
OTTHUMP00000067378 antibody

Accession NoSwiss-Prot#:P19022

Uniprot P19022

Gene ID 1000;

Calculated MW120/140 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of N Cadherin on different lysates using anti-N Cadherin antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: Mouse brain
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.
ICC staining N Cadherin in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining N Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining N Cadherin in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining N Cadherin in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with N Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy-terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5.

If you have published an article using product 48779, please notify us so that we can cite your literature.

et al,PD-L1 Expression Is Regulated By NF-κB DurIng EMT SignalIng In Gastric CarcInoma. In Onco Targets Ther on 2019 Nov 25 by Xu D, Li J, et al..PMID:31819504 , (2019),
PMID: 31819504

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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