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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Smad1 Rabbit mAb

Smad1 Rabbit mAb#48784

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Product Detail

Product NameSmad1 Rabbit mAb

Clone No.SY0254

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesBSP-1 antibody
BSP1 antibody
HsMAD1 antibody
JV4-1 antibody
JV41 antibody
MAD homolog 1 antibody
MAD mothers against decapentaplegic homolog 1 antibody
Mad related protein 1 antibody
Mad-related protein 1 antibody
MADH1 antibody
MADR1 antibody
Mothers against decapentaplegic homolog 1 antibody
Mothers against DPP homolog 1 antibody
SMA- AND MAD-RELATED PROTEIN 1 antibody
SMAD 1 antibody
SMAD family member 1 antibody
SMAD mothers against DPP homolog 1 antibody
Smad1 antibody
SMAD1_HUMAN antibody
TGF beta signaling protein 1 antibody
Transforming growth factor-beta-signaling protein 1 antibody

Accession NoSwiss-Prot#:Q15797

Uniprot Q15797

Gene ID 4086;

Calculated MW52 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1000

ICC: 1:100-1:500

ICC staining Smad1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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