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alpha smooth muscle Actin Rabbit mAb#48785

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Product Namealpha smooth muscle Actin Rabbit mAb

Clone No.SY25-03

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, FC,IP

Species ReactivityHu, Ms, Rt, zebrafish

Immunogen TypeRecombinant Protein

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namesa actin antibody
AAT6 antibody
ACTA_HUMAN antibody
ACTA2 antibody
Actin alpha 2 smooth muscle aorta antibody
Actin aortic smooth muscle antibody
Actin, aortic smooth muscle antibody
ACTSA antibody
ACTVS antibody
Alpha 2 actin antibody
Alpha actin 2 antibody
Alpha cardiac actin antibody
Alpha-actin-2 antibody
Cell growth inhibiting gene 46 protein antibody
Cell growth-inhibiting gene 46 protein antibody
GIG46 antibody
Growth inhibiting gene 46 antibody
MYMY5 antibody

Accession NoSwiss-Prot#:P62736

Uniprot P62736

Gene ID 59;

Calculated MW42 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-5,000
IHC:1:50-1:200


FC: 1:50-1:100

Western blot analysis of alpha smooth muscle Actin on different lysates using anti-alpha smooth muscle Actin antibody at 1/1,000 dilution. Positive control:
Lane 1: A549
Lane 2: Hela
Lane 3: NIH/3T3
Lane 4: Mouse heart
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with alpha smooth muscle Actin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas Cβ and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

If you have published an article using product 48785, please notify us so that we can cite your literature.

et al,Novel therapeutic insights into pathological cardiac hypertrophy: tRF-16-R29P4PE regulates PACE4 and metabolic pathways. , (2025),
PMID: 39947523

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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