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Location： Home > Recombinant Rabbit Monoclonal Antibodies > DARPP32 Rabbit mAb

DARPP32 Rabbit mAb#48815

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Product Detail

Product NameDARPP32 Rabbit mAb

Clone No.SU0329

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesDARPP 32 antibody
DARPP-32 antibody
Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
Dopamine and cAMP regulated neuronal phosphoprotein antibody
Dopamine and cAMP regulated phosphoprotein antibody
Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
FLJ20940 antibody
IPPD antibody
Neuronal phosphoprotein DARPP 32 antibody
PPP1R1B antibody
PPR1B_HUMAN antibody
Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
Protein phosphatase 1 regulatory subunit 1B antibody

Accession NoSwiss-Prot#:Q9UD71

Uniprot Q9UD71

Gene ID 84152;

Calculated MW32 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
ICC staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.

If you have published an article using product 48815, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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