Product Detail
Product NameCyclin B1 Rabbit mAb
Clone No.SU33-03
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, IHC, IP
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesCCNB 1 antibody CCNB antibody ccnb1 antibody CCNB1_HUMAN antibody Cyclin B1 antibody G2 mitotic specific cyclin B1 antibody G2/mitotic-specific cyclin-B1 antibody
Accession NoSwiss-Prot#:P14635
Uniprot
P14635
Gene ID
891;
Calculated MW55 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of Cyclin B1 on different lysates using anti-Cyclin B1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Daudi Lane 3: K562
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin B1 antibody. Counter stained with hematoxylin.
In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme; tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.
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