Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Mouse Monoclonal Antibodies > CD20 mouse mAb

CD20 mouse mAb#48819

Availability: Yes
Total Amount:$
Product Detail

Product NameCD20 mouse mAb

Clone No.SR4346

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1, Kappa

PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

ApplicationsIHC;WB;IF

Species ReactivityHuman

SpecificityThe antibody can specifically recognize human CD20 protein.

Immunogen DescSynthesized peptide derived from human CD20

Target NameMS4A1 CD20

ConjugateUnconjugated

Other NamesB-lymphocyte antigen CD20 (B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 1;CD antigen CD20)

Uniprot P11836

Gene ID 931

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.84% sodium azide.

StorageStore at -20℃/1 year

Application Details

 IHC-p 1:100-500, WB 1:200-1000, IF 1:100-500

 
All lanes : CD20 Mouse mAb at 1/1k dilution
Lane 1 : Daudi cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 37 kDa
Exposure time: 6 seconds
Human tonsil tissue was stained with anti-CD20 antibody.
Immunocytochemistry/ Immunofluorescence CD20 antibody (48819)

ICC/IF staining of CD20 antibody in K562 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48819 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti mouse, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 48819, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;