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Location： Home > Recombinant Rabbit Monoclonal Antibodies > AMPK alpha 1 Rabbit mAb

AMPK alpha 1 Rabbit mAb#48827

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Product Detail

Product NameAMPK alpha 1 Rabbit mAb

Clone No.SU03-48

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names5 AMP activated protein kinase alpha 1catalytic subunit antibody
5 AMP activated protein kinase catalytic alpha 1 chain antibody
5' AMP activated protein kinase catalytic subunit alpha 1 antibody
5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
AAPK1 antibody
AAPK1_HUMAN antibody
ACACA kinase antibody
acetyl CoA carboxylase kinase antibody
AI194361 antibody
AI450832 antibody
AL024255 antibody
AMP -activate kinase alpha 1 subunit antibody
AMP-activated protein kinase, catalytic, alpha -1 antibody
AMPK 1 antibody
AMPK alpha 1 antibody
AMPK alpha 1 chain antibody
AMPK antibody
AMPK subunit alpha-1 antibody
AMPK1 antibody
AMPKa1 antibody
AMPKalpha1 antibody
C130083N04Rik antibody
cb116 antibody
EC 2.7.11.1 antibody
HMG CoA reductase kinase antibody
HMGCR kinase antibody
hormone sensitive lipase kinase antibody
Hydroxymethylglutaryl CoA reductase kinase antibody
im:7154392 antibody
kinase AMPK alpha1 antibody
MGC33776 antibody
MGC57364 antibody
OTTHUMP00000161795 antibody
OTTHUMP00000161796 antibody
PRKAA 1 antibody
PRKAA1 antibody
Protein kinase AMP activated alpha 1 catalytic subunit antibody
SNF1-like protein AMPK antibody
SNF1A antibody
Tau protein kinase PRKAA1 antibody
wu:fa94c10 antibody

Accession NoSwiss-Prot#:Q13131

Uniprot Q13131

Gene ID 5562;

Calculated MW63 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000

ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of AMPK alpha 1 on different lysates using anti-AMPK alpha 1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: MCF-7
ICC staining AMPK alpha 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AMPK alpha 1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AMPK alpha 1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with AMPK alpha 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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