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Location： Home > Recombinant Rabbit Monoclonal Antibodies > ASK1 Rabbit mAb

ASK1 Rabbit mAb#48837

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Product Detail

Product NameASK1 Rabbit mAb

Clone No.SU36-06

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesApoptosis signal regulating kinase 1 antibody
Apoptosis signal-regulating kinase 1 antibody
ASK 1 antibody
ASK-1 antibody
ASK1 antibody
M3K5 antibody
M3K5_HUMAN antibody
MAP/ERK kinase kinase 5 antibody
MAP3K5 antibody
MAPK/ERK kinase kinase 5 antibody
MAPKKK5 antibody
MEK kinase 5 antibody
MEKK 5 antibody
MEKK5 antibody
Mitogen activated protein kinase kinase kinase 5 antibody
Mitogen-activated protein kinase kinase kinase 5 antibody

Accession NoSwiss-Prot#:Q99683

Uniprot Q99683

Gene ID 4217;

Calculated MW155 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
ICC staining ASK1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ASK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ASK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with ASK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.

If you have published an article using product 48837, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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