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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PARP Rabbit mAb

PARP Rabbit mAb#48838

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Product Detail

Product NamePARP Rabbit mAb

Clone No.SU03-68

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
ADP ribosyltransferase diphtheria toxin like 1 antibody
ADP ribosyltransferase NAD(+) antibody
ADPRT 1 antibody
ADPRT antibody
ADPRT1 antibody
ARTD1 antibody
msPARP antibody
NAD(+) ADP ribosyltransferase 1 antibody
NAD(+) ADP-ribosyltransferase 1 antibody
pADPRT 1 antibody
pADPRT1 antibody
PARP 1 antibody
PARP antibody
PARP-1 antibody
PARP1 antibody
PARP1_HUMAN antibody
Poly (ADP ribose) polymerase 1 antibody
poly (ADP ribose) polymerase family, member 1 antibody
Poly [ADP-ribose] polymerase 1 antibody
Poly(ADP ribose) polymerase antibody
poly(ADP ribose) synthetase antibody
poly(ADP ribosyl)transferase antibody
Poly[ADP ribose] synthetase 1 antibody
Poly[ADP-ribose] synthase 1 antibody
PPOL antibody

Accession NoSwiss-Prot#:P09874

Uniprot P09874

Gene ID 142;

Calculated MW113/89 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of PARP on different lysates using anti-PARP antibody at 1/1,000 dilution. Positive control:
Lane 1: A549
Lane 2: Jurkat
Lane 3: Hela
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. Counter stained with hematoxylin.
ICC staining PARP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

If you have published an article using product 48838, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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