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Recombinant Rabbit Monoclonal Antibodies > Calreticulin Rabbit mAb
Calreticulin Rabbit mAb#48841
Yes
All lanes: Calreticulin Rabbit mAb at 1/1k dilution
Lane 1 : HUVEC whole cell lysates
Lane 2 : A549 whole cell lysatesLane 3 : Raw264.7 whole cell lysatesLane 4 : 3T3 whole cell lysatesLane 5 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa
Exposure time: 6 seconds
All lanes :Calreticulin Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Calreticulin knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver tissue stained for Calreticulin using 48841 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Calreticulin using 48841 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse brain tissue stained for Calreticulin using 48841 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Calreticulin antibody (48841) ICC/IF staining of Calreticulin in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 48841 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Calreticulin antibody (48841) ICC/IF staining of Calreticulin in NIH/3T3 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 48841 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
Flow Cytometry (Intracellular) Calreticulin antibody (48841)
Flow cytometric analysis of Hela cells with Calreticulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody，black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
