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Location： Home > Recombinant Rabbit Monoclonal Antibodies > p27 KIP 1 Rabbit mAb

p27 KIP 1 Rabbit mAb#48842

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Product Detail

Product Namep27 KIP 1 Rabbit mAb

Clone No.SU37-04

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAA408329 antibody
AI843786 antibody
Cdki1b antibody
CDKN 1B antibody
CDKN 4 antibody
CDKN1B antibody
CDKN4 antibody
CDN1B_HUMAN antibody
Cyclin Dependent Kinase Inhibitor 1B antibody
Cyclin dependent kinase inhibitor p27 antibody
Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody
Cyclin-dependent kinase inhibitor 1B antibody
Cyclin-dependent kinase inhibitor p27 antibody
Cyclin-dependent kinase inhibitor p27 Kip1 antibody
KIP 1 antibody
KIP1 antibody
MEN1B antibody
MEN4 antibody
OTTHUMP00000195098 antibody
OTTHUMP00000195099 antibody
p27 antibody
p27 Kip1 antibody
P27-like cyclin-dependent kinase inhibitor antibody
p27Kip1 antibody

Accession NoSwiss-Prot#:P46527

Uniprot P46527

Gene ID 1027;

Calculated MW27 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:100-1:500
ICC: 1:100-1:500

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
ICC staining p27 KIP 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining p27 KIP 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with p27 KIP 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Cell cycle progression is regulated by a series of cyclin-dependent kinases consisting of catalytic subunits, designated Cdks, as well as activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has recently been described that function as "mitotic inhibitors." These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and a more recently described p16-related inhibitor designated p15. A p21-related protein, p27, has been described as a negative regulator of G1 progression and speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and, to a lesser extent, with cyclin E and Cdk2.

If you have published an article using product 48842, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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