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Location： Home > Recombinant Rabbit Monoclonal Antibodies > p16INK4A Rabbit mAb

p16INK4A Rabbit mAb#48843

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Product Detail

Product Namep16INK4A Rabbit mAb

Clone No.SU0702

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCCM2 antibody
CDK4 inhibitor p16 INK4 antibody
CDK4I antibody
CDKN2 antibody
CDKN2A antibody
Cell cycle negative regulator beta antibody
CMM2 antibody
Cyclin dependent kinase 4 inhibitor A antibody
Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
Cyclin Dependent Kinase Inhibitor 2A antibody
Cyclin dependent kinase inhibitor 2A isoform 4 antibody
Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
Cyclin dependent kinase inhibitor p16 antibody
INK4 antibody
INK4A antibody
MLM antibody
MTS1 antibody
Multiple tumor suppressor 1 antibody
p14 antibody
p16 antibody
P16INK4 antibody
p16INK4a antibody
p19 antibody
p19Arf antibody
TP16 antibody

Accession NoSwiss-Prot#:P42771

Uniprot P42771

Gene ID 1029;

Calculated MW17 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.
ICC staining p16INK4A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining p16INK4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining p16INK4A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates, promote orderly progression through the cell cycle. The protein p16INK4A, identified as a negative regulator of the cell cycle, has been shown to bind to and inhibit the activity of the Cdk4/cyclin D complex. p19 ARF, which is unrelated to p16, arises from transcription of an alternative reading frame of the p16 gene. Like p16, p19 ARF has been shown to induce cell cycle arrest. Mice lacking p19 ARF but expressing functional p16 have been shown to develop tumors early in life. Further studies have indicated that p19 ARF may be disrupted in a large percentage of human T cell acute lymphoblastic leukemias.

If you have published an article using product 48843, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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