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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PI 3 Kinase p85 alpha Rabbit mAb

PI 3 Kinase p85 alpha Rabbit mAb#48848

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Product NamePI 3 Kinase p85 alpha Rabbit mAb

Clone No.SU04-07

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB IHC ICC/IF

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic peptide within C-terminal human PI 3 Kinase p85 alpha.

ConjugateUnconjugated

Other NamesGRB1 antibody
p85 alpha antibody
p85 antibody
P85A_HUMAN antibody
Phosphatidylinositol 3 kinase associated p 85 alpha antibody
Phosphatidylinositol 3 kinase regulatory 1 antibody
Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
PI3 kinase p85 subunit alpha antibody
PI3-kinase regulatory subunit alpha antibody
PI3-kinase subunit p85-alpha antibody
PI3K antibody
PI3K regulatory subunit alpha antibody
Pik3r1 antibody
PtdIns 3 kinase p85 alpha antibody
PtdIns-3-kinase regulatory subunit alpha antibody
PtdIns-3-kinase regulatory subunit p85-alpha antibody

Accession NoSwiss-Prot#:P27986

Uniprot P27986

Gene ID 5295;

Calculated MWPredicted band size: 84 kDa

Sdspage MWObserved band size: 84 kDa

Concentration1 mg/mL

Formulation1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: PI 3 Kinase p85 alpha Rabbit mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 84 kDa
Observed band size: 84 kDa

Exposure time: 4 seconds
All lanes :PI 3 Kinase p85 alpha Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : PI 3 Kinase p85 alpha knockdown A549 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human placenta tissue stained for PI 3 Kinase p85 alpha using 48848 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse testis tissue stained for PI 3 Kinase p85 alpha using 48848 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence PI 3 Kinase p85 alpha antibody (48848)

ICC/IF staining of PI 3 Kinase p85 alpha in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48848 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Phosphatidylinositol 3-kinase (PI 3-kinase) phosphorylates the 3' OH position of the inositol ring of inositol lipids and is composed of p85 and p110 subunits. PI 3-kinase p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. PI 3-kinase p85α, also known as GRB1, phosphatidylinositol 3-kinase regulatory 1 or p85, is a 724 amino acid protein that exists as four alternatively spliced isoforms. Involved in insulin metabolism, defects in the PI 3-kinase p85α gene have been linked to insulin resistance. PI 3-kinase p85α is polyubiquitinated in T-cells by Cbl-b, and has multiple phosphorylated amino acid residues, including a phosphorylated tyrosine residue at position 467.

If you have published an article using product 48848, please notify us so that we can cite your literature.

et al,Effect of Fushengong Decoction on PTEN/PI3K/AKT/NF-KB Pathway in Rats With Chronic Renal Failure via Dual-Dimension Network Pharmacology Strategy.In Front Pharmacol on 2022 Mar 15 by Hongyu Luo, Munan Wang,et al..PMID: 35370667 , (2022),
PMID: 35370667
et al,Effect of Fushengong Decoction on PTEN/PI3K/AKT/NF-κB Pathway in Rats With Chronic Renal Failure via Dual-Dimension Network Pharmacology Strategy. In Front Pharmacol on 2022 Mar 15 by Hongyu Luo, Munan Wang, et al..PMID: 35370667 , (2022),
PMID: 35370667

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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