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Location： Home > Recombinant Rabbit Monoclonal Antibodies > YB1 Rabbit mAb

YB1 Rabbit mAb#48851

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Product Detail

Product NameYB1 Rabbit mAb

Clone No.ST0432

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesBP 8 antibody
CBF-A antibody
CCAAT binding transcription factor I subunit A antibody
CCAAT-binding transcription factor I subunit A antibody
CSDA2 antibody
CSDB antibody
DBPB antibody
DNA binding protein B antibody
DNA-binding protein B antibody
EFI-A antibody
Enhancer factor I subunit A antibody
MDR NF1 antibody
MGC104858 antibody
MGC110976 antibody
MGC117250 antibody
NSEP 1 antibody
NSEP1 antibody
Nuclease sensitive element binding protein 1 antibody
Nuclease-sensitive element-binding protein 1 antibody
p50 antibody
Q15905 antibody
Y-box binding protein 1 antibody
Y-box transcription factor antibody
Y-box-binding protein 1 antibody
YB 1 antibody
YB-1 antibody
YBOX1_HUMAN antibody
YBX 1 antibody
ybx1 antibody

Accession NoSwiss-Prot#:P67809

Uniprot P67809

Gene ID 4904;

Calculated MW50 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Jurkat
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-YB1 antibody. Counter stained with hematoxylin.
ICC staining YB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining YB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining YB1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Y-box binding protein YB-1 (also known as CCAAT-binding transcription factor, enhancer factor I subunit A and DNA-binding protein B) belongs to a family of multifunctional proteins which regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing Arginine clusters and aromatic residues. YB-1 plays a role in cell proliferation as an activator of growth-associated gene expression. YB-1 is also a repressor of the cell death-associated gene FAS. YB-1 may play an important role in controlling cell survival by regulating the expression of cell growth-associated and death-associated genes.

If you have published an article using product 48851, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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