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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PIM1 Rabbit mAb

PIM1 Rabbit mAb#48894

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Product Detail

Product NamePIM1 Rabbit mAb

Clone No.ST0513

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesOncogene PIM 1 antibody
Oncogene PIM1 antibody
PIM 1 antibody
pim 1 kinase 44 kDa isoform antibody
Pim 1 kinase antibody
pim 1 oncogene (proviral integration site 1) antibody
Pim 1 oncogene antibody
PIM antibody
PIM1 antibody
pim1 kinase 44 kDa isoform antibody
PIM1_HUMAN antibody
Pim2 antibody
PIM3 antibody
Proto oncogene serine/threonine protein kinase Pim 1 antibody
Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
Proviral integration site 1 antibody
Proviral integration site 2 antibody

Accession NoSwiss-Prot#:P11309

Uniprot P11309

Gene ID 5292;

Calculated MW32 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of PIM1 on different lysates using anti-PIM1 antibody at 1/1,000 dilution. Positive control:
Lane 1: BT-20
Lane 2: HepG2
Lane 3: SW480
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
ICC staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pim-1 is a serine/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of pim-1 increases during the progression from early to late G1, remaining high at the G1/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases.

If you have published an article using product 48894, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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